[Shalika, Katsuki]

Both hydrogel and force gauge are on translation stage. We moved the force gauge up using the actuator on translation stage.See Fig 1 and Fig 2.

hydrogel is 8-9cm in length and 1.5mm thick. Each measurement take 2:30 mins

The laser is at center

0.26 N

C:\Users\atama\Dropbox\Cell Birefringence\Measurement data\hydrogel\Force gauge v2\Fri, Oct 25, 2024 4-02-01 PM.txt

0.28

C:\Users\atama\Dropbox\Cell Birefringence\Measurement data\hydrogel\Force gauge v2\Fri, Oct 25, 2024 4-06-13 PM.txt

0.30

C:\Users\atama\Dropbox\Cell Birefringence\Measurement data\hydrogel\Force gauge v2\Fri, Oct 25, 2024 4-09-53 PM.txt

0.32 N

C:\Users\atama\Dropbox\Cell Birefringence\Measurement data\hydrogel\Force gauge v2\Fri, Oct 25, 2024 4-13-41 PM.txt

0.34 N

C:\Users\atama\Dropbox\Cell Birefringence\Measurement data\hydrogel\Force gauge v2\Fri, Oct 25, 2024 4-18-30 PM.txt

0.36

C:\Users\atama\Dropbox\Cell Birefringence\Measurement data\hydrogel\Force gauge v2\Fri, Oct 25, 2024 4-22-30 PM.txt

0.38

C:\Users\atama\Dropbox\Cell Birefringence\Measurement data\hydrogel\Force gauge v2\Fri, Oct 25, 2024 4-26-43 PM.txt

0.40

C:\Users\atama\Dropbox\Cell Birefringence\Measurement data\hydrogel\Force gauge v2\Fri, Oct 25, 2024 4-30-35 PM.txt

0.42 N

C:\Users\atama\Dropbox\Cell Birefringence\Measurement data\hydrogel\Force gauge v2\Fri, Oct 25, 2024 4-34-02 PM.txt

0.44

C:\Users\atama\Dropbox\Cell Birefringence\Measurement data\hydrogel\Force gauge v2\Fri, Oct 25, 2024 4-37-43 PM.txt

0.46

Fri, Oct 25, 2024 4-41-56 PM

0.48

C:\Users\atama\Dropbox\Cell Birefringence\Measurement data\hydrogel\Force gauge v2\Fri, Oct 25, 2024 4-46-00 PM.txt

0.50

C:\Users\atama\Dropbox\Cell Birefringence\Measurement data\hydrogel\Force gauge v2\Fri, Oct 25, 2024 4-50-26 PM.txt

we moved the hydrogel laterally--the beam is not in center

0.51

C:\Users\atama\Dropbox\Cell Birefringence\Measurement data\hydrogel\Force gauge v2\Fri, Oct 25, 2024 4-54-17 PM.txt

0.48

C:\Users\atama\Dropbox\Cell Birefringence\Measurement data\hydrogel\Force gauge v2\Fri, Oct 25, 2024 4-59-40 PM.txt

0.46

C:\Users\atama\Dropbox\Cell Birefringence\Measurement data\hydrogel\Force gauge v2\Fri, Oct 25, 2024 5-03-11 PM.txt

0.44

C:\Users\atama\Dropbox\Cell Birefringence\Measurement data\hydrogel\Force gauge v2\Fri, Oct 25, 2024 5-06-56 PM.txt

0.42

C:\Users\atama\Dropbox\Cell Birefringence\Measurement data\hydrogel\Force gauge v2\Fri, Oct 25, 2024 5-11-10 PM.txt

0.40

C:\Users\atama\Dropbox\Cell Birefringence\Measurement data\hydrogel\Force gauge v2\Fri, Oct 25, 2024 5-17-25 PM.txt

0.38

C:\Users\atama\Dropbox\Cell Birefringence\Measurement data\hydrogel\Force gauge v2\Fri, Oct 25, 2024 5-21-26 PM.txt

0.36

C:\Users\atama\Dropbox\Cell Birefringence\Measurement data\hydrogel\Force gauge v2\Fri, Oct 25, 2024 5-24-52 PM.txt

0.34

C:\Users\atama\Dropbox\Cell Birefringence\Measurement data\hydrogel\Force gauge v2\Fri, Oct 25, 2024 5-29-02 PM.txt

0.32

C:\Users\atama\Dropbox\Cell Birefringence\Measurement data\hydrogel\Force gauge v2\Fri, Oct 25, 2024 5-32-51 PM.txt

The hydrogel maybe is a bit dry. So, the 0.32 was the minimum the translation stage could move.

We moved the hydrogel laterally to another side.

0.32

C:\Users\atama\Dropbox\Cell Birefringence\Measurement data\hydrogel\Force gauge v2\Fri, Oct 25, 2024 5-41-43 PM.txt

0.36

C:\Users\atama\Dropbox\Cell Birefringence\Measurement data\hydrogel\Force gauge v2\Fri, Oct 25, 2024 5-45-22 PM.txt

we stop coz the force change by itself.